Anti-Ricin toxin A chain antibody (ab27169)
Key features and details
- Rabbit polyclonal to Ricin toxin A chain
- Suitable for: ELISA, WB, Electron Microscopy
- Reacts with: Castor bean
- Isotype: IgG
Overview
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Product name
Anti-Ricin toxin A chain antibody -
Description
Rabbit polyclonal to Ricin toxin A chain -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, Electron Microscopymore details -
Species reactivity
Reacts with: Castor bean -
Immunogen
Full length native protein: Purified intact A chain from RCA 60.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Whole antiserum -
Purification notes
sterile-filtered (0.22 mm pore size) serum with the addition of 15 mM NaN3 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/20000.
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WB |
1/2000. Predicted molecular weight: 32 kDa.
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Electron Microscopy |
Use at an assay dependent concentration. PubMed: 21886775
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Notes |
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ELISA
1/20000. |
WB
1/2000. Predicted molecular weight: 32 kDa. |
Electron Microscopy
Use at an assay dependent concentration. PubMed: 21886775 |
Target
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Relevance
Ricin chain A (RTA) is a ribosome inactivating enzyme with a molecular weight of 32 kDa. RTA is normally linked by a disulfide bond to the galactose/N acetylgalactosamine binding lectin (34 kDa), also called the B chain or RTB. Together with RTB, RTA constitutes the heterodimeric cytotoxin Ricin (RCA 60) from Ricinus communis. This ricin, which is a type 2 RIP (ribosome inactivating protein) is among the most potent cytotoxins in nature. Ricin is highly toxic to animal cells and to a lesser extent to plant cells. -
Alternative names
- Ricin A chain antibody
- Ricin antibody
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab27169 has been referenced in 4 publications.
- Marszalowicz GP et al. GUCY2C lysosomotropic endocytosis delivers immunotoxin therapy to metastatic colorectal cancer. Oncotarget 5:9460-71 (2014). PubMed: 25294806
- Wang W et al. Mannosidase 2, alpha 1 Deficiency Is Associated with Ricin Resistance in Embryonic Stem (ES) Cells. PLoS One 6:e22993 (2011). Electron Microscopy . PubMed: 21886775
- Ray S et al. Modulation of toxin stability by 4-phenylbutyric Acid and negatively charged phospholipids. PLoS One 6:e23692 (2011). PubMed: 21887297
- Yin HQ et al. A nanoparticle-based bio-barcode assay for ultrasensitive detection of ricin toxin. Toxicon : (2011). PubMed: 22005297