Anti-Ricinus communis agglutinin 60 antibody [CP75] (ab20968)

Overview

  • Product nameAnti-Ricinus communis agglutinin 60 antibody [CP75]
  • Description
    Mouse monoclonal [CP75] to Ricinus communis agglutinin 60
  • SpecificityRecognizes the A chain of RCA60. Also recognizes RCA120.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Ricinus communis and Rice (see Abreview).
  • Immunogen

    Natural RCA60, full length.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberCP75
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 63 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceRicinus communis agglutinin 60(RCA60) is an extremely toxic glycoprotein consisting of two disulfide-linked chains of about 28,000 daltons and 32,000 daltons, termed A and B chain, respectively. The B chain can bind to cell surfaces via galactose or N-acetylgalactosamine residues of membrane glycoconjugates and facilitates the transport of the lectin into the cell. The A chain has an enzyme activity which can catalytically block protein synthesis and is so toxic that only a single molecule of A chain is required to kill a cell. Alone, the A chain is incapable of entering the cell and is thus not toxic. The A chain can be separated from the B chain and then linked by a cleavable disulfide bond to other proteins, such as antibodies. These antibody-toxin hybrid molecules can be used to kill specific cells with the appropriate antigenic determinant exposed, such as tumor markers on surfaces of malignant cells.
    • Alternative names
      • Agglutinin A chain antibody
      • Agglutinin B chain antibody
      • Castor bean antibody
      • RCA 60 antibody
      • RCA60 antibody
      • Ricin A chain antibody
      • Ricin antibody
      • Ricin B chain antibody
      see all

    References for Anti-Ricinus communis agglutinin 60 antibody [CP75] (ab20968)

    This product has been referenced in:
    • Maerz S  et al. The Neurospora peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity. J Biol Chem 285:2326-32 (2010). WB . Read more (PubMed: 19940117) »
    • Hosomi A  et al. Identification of an Htm1 (EDEM)-dependent, Mns1-independent Endoplasmic Reticulum-associated Degradation (ERAD) Pathway in Saccharomyces cerevisiae: APPLICATION OF A NOVEL ASSAY FOR GLYCOPROTEIN ERAD. J Biol Chem 285:24324-34 (2010). WB . Read more (PubMed: 20511219) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rice Recombinant protein (E. coli produced protein)
    Specification E. coli produced protein
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. Linda McLoon

    Verified customer

    Submitted Feb 14 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"