The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 63 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
RelevanceRicinus communis agglutinin 60(RCA60) is an extremely toxic glycoprotein consisting of two disulfide-linked chains of about 28,000 daltons and 32,000 daltons, termed A and B chain, respectively. The B chain can bind to cell surfaces via galactose or N-acetylgalactosamine residues of membrane glycoconjugates and facilitates the transport of the lectin into the cell. The A chain has an enzyme activity which can catalytically block protein synthesis and is so toxic that only a single molecule of A chain is required to kill a cell. Alone, the A chain is incapable of entering the cell and is thus not toxic. The A chain can be separated from the B chain and then linked by a cleavable disulfide bond to other proteins, such as antibodies. These antibody-toxin hybrid molecules can be used to kill specific cells with the appropriate antigenic determinant exposed, such as tumor markers on surfaces of malignant cells.
Agglutinin A chain antibody
Agglutinin B chain antibody
Castor bean antibody
RCA 60 antibody
Ricin A chain antibody
Ricin B chain antibody
References for Anti-Ricinus communis agglutinin 60 antibody [CP75] (ab20968)
This product has been referenced in:
Maerz S et al. The Neurospora peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity. J Biol Chem285:2326-32 (2010).
Read more (PubMed: 19940117) »
Hosomi A et al. Identification of an Htm1 (EDEM)-dependent, Mns1-independent Endoplasmic Reticulum-associated Degradation (ERAD) Pathway in Saccharomyces cerevisiae: APPLICATION OF A NOVEL ASSAY FOR GLYCOPROTEIN ERAD. J Biol Chem285:24324-34 (2010).
Read more (PubMed: 20511219) »