• Product nameAnti-RICTOR antibody [7B3]
    See all RICTOR primary antibodies
  • Description
    Mouse monoclonal [7B3] to RICTOR
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Purified recombinant fragment of Human RICTOR expressed in E. Coli.

  • Positive control
    • HeLa, PANC-1, MOLT4, HepG2 and HEK293 cell lysates. Thyroid gland tissues and Human breast carcinoma. NIH3T3 cells.



Our Abpromise guarantee covers the use of ab104838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 192 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.


  • FunctionSubunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
  • Sequence similaritiesBelongs to the RICTOR family.
  • Post-translational
    Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • AVO3 antibody
    • AVO3 homolog antibody
    • DKFZp686B11164 antibody
    • hAVO3 antibody
    • KIAA1999 antibody
    • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
    • mAVO3 antibody
    • MGC39830 antibody
    • PIA antibody
    • Pianissimo antibody
    • Rapamycin insensitive companion of mTOR antibody
    • Rapamycin-insensitive companion of mTOR antibody
    • Rictor antibody
    • RICTR antibody
    • RICTR_HUMAN antibody
    • RPTOR independent companion of MTOR complex 2 antibody
    • TORC2 specific protein AVO3 antibody
    see all

Anti-RICTOR antibody [7B3] images

  • All lanes : Anti-RICTOR antibody [7B3] (ab104838) at 1/500 dilution

    Lane 1 : HeLa cell lysate.
    Lane 2 : PANC-1 cell lysate.
    Lane 3 : MOLT4 cell lysate.
    Lane 4 : HepG2 cell lysate.
    Lane 5 : HEK293 cell lysate.

    Predicted band size : 192 kDa
  • ab104838 at 1/200 dilution staining RICTOR in NIH3T3 cells (green) by immunofluorescence. DRAQ5 fluorescent DNA dye is shown in blue. Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-embedded thyroid gland tissues by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-fixed Human breast carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 used for Flow cytometric analysis of HeLa cells (green). The negative control is shown in purple.

References for Anti-RICTOR antibody [7B3] (ab104838)

This product has been referenced in:
  • Hütt-Cabezas M  et al. Activation of mTORC1/mTORC2 signaling in pediatric low-grade glioma and pilocytic astrocytoma reveals mTOR as a therapeutic target. Neuro Oncol 15:1604-14 (2013). IHC-P ; Human . Read more (PubMed: 24203892) »

See 1 Publication for this product

Product Wall

Thank you very much for taking the time to complete and send the questionnaire.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

Unfortunately none of these antibodie...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

Read More