Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human RICTOR.
Our Abpromise guarantee covers the use of ab105469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: RICTOR knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab105469 observed at 192 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab105469 was shown to recognize RICTOR in wild-type HAP1 cells as signal was lost at the expected MW in RICTOR knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RICTOR knockout samples were subjected to SDS-PAGE. ab105469 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 µg/mL and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab105469 has not yet been referenced specifically in any publications.