Overview

  • Product nameAnti-RICTOR antibody
    See all RICTOR primary antibodies
  • Description
    Rabbit polyclonal to RICTOR
  • SpecificityInitial tests for reactivity with mouse RICTOR from NIH3T3 cells suggest that higher amounts of lysate and less dilution of ab70374 are required for brief exposure times.
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1650 and the C-terminus (residue 1708) of human RICTOR.

  • Positive control
    • Whole cell lysates from HeLa and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
IP Use at 2-10 µg/mg of lysate.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5µg for 106 cells.

0.5 μg per 1 million cells in a 150 mcl reaction.





ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionSubunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
  • Sequence similaritiesBelongs to the RICTOR family.
  • Post-translational
    modifications
    Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • AVO3 antibody
    • AVO3 homolog antibody
    • DKFZp686B11164 antibody
    • hAVO3 antibody
    • KIAA1999 antibody
    • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
    • mAVO3 antibody
    • MGC39830 antibody
    • PIA antibody
    • Pianissimo antibody
    • Rapamycin insensitive companion of mTOR antibody
    • Rapamycin-insensitive companion of mTOR antibody
    • Rictor antibody
    • RICTR antibody
    • RICTR_HUMAN antibody
    • RPTOR independent companion of MTOR complex 2 antibody
    • TORC2 specific protein AVO3 antibody
    see all

Anti-RICTOR antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling RICTOR with ab70374 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RICTOR antibody (ab70374) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 5 µg
    Lane 2 : HeLa whole cell lysate at 2.5 µg
    Lane 3 : HeLa whole cell lysate at 1 µg
    Lane 4 : HeLa whole cell lysate at 0.5 µg
    Lane 5 : NIH3T3 cells at 50 µg


    Predicted band size : 192 kDa
    Observed band size : 192 kDa
    Additional bands at : 78 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG.
  • ab70374 (2µg/ml) staining RICTOR in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of glandular epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-RICTOR antibody (ab70374)

This product has been referenced in:
  • Li J  et al. Rictor/mTORC2 protects against cisplatin-induced tubular cell death and acute kidney injury. Kidney Int N/A:N/A (2014). Read more (PubMed: 24451322) »
  • Takai H  et al. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev 24:2019-30 (2010). IP ; Mouse . Read more (PubMed: 20801936) »

See all 2 Publications for this product

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