The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
Use at 2-10 µg/mg of lysate.
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use 0.5µg for 106 cells.
0.5 μg per 1 million cells in a 150 mcl reaction.
ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSubunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Sequence similaritiesBelongs to the RICTOR family.
Post-translational modificationsPhosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling RICTOR with ab70374 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - RICTOR antibody (ab70374)
All lanes : Anti-RICTOR antibody (ab70374) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg Lane 2 : HeLa whole cell lysate at 2.5 µg Lane 3 : HeLa whole cell lysate at 1 µg Lane 4 : HeLa whole cell lysate at 0.5 µg Lane 5 : NIH3T3 cells at 50 µg
Predicted band size : 192 kDa Observed band size : 192 kDa Additional bands at : 78 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - RICTOR antibody (ab70374)
Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG.
ab70374 (2µg/ml) staining RICTOR in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of glandular epithelium. Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-RICTOR antibody (ab70374)
This product has been referenced in:
Li J et al. Rictor/mTORC2 protects against cisplatin-induced tubular cell death and acute kidney injury. Kidney IntN/A:N/A (2014).
Read more (PubMed: 24451322) »
Takai H et al. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev24:2019-30 (2010).
Read more (PubMed: 20801936) »