- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-Rif1 antibody
See all Rif1 primary antibodies - DescriptionRabbit polyclonal to Rif1
- SpecificityThe immunogen sequence for ab13422 represents the C-terminal end of mouse Rif1, mouse teleomere-associated protein RIF1 and mouse Rap1-interacting factor.
- Tested applicationsSuitable for: IP, ELISA, WBmore details
- Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Dog, Human
- Immunogen
Synthetic peptide:
C-RNLQSRWRSPAHENS
conjugated to KLH, corresponding to C terminal amino acids 531-545 of Mouse Rif1.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab13422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | ||
| ELISA | ||
| WB |
IP: Use at an assay dependent dilution (PMID 19000314).
WB: 1/500 - 1/3000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionRequired for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
- Tissue specificityHighly expressed in testis.
- Developmental stageExpression peaks in late G2/S phase of the cell cycle.
- Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Cellular localizationNucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
- Information by UniProt
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Database links
- Entrez Gene: 541247 Cow
- Entrez Gene: 609234 Dog
- Entrez Gene: 55183 Human
- Entrez Gene: 51869 Mouse
- Entrez Gene: 295602 Rat
- Omim: 608952 Human
- SwissProt: Q5UIP0 Human
- SwissProt: Q6PR54 Mouse
see all -
Alternative names
- DKFZp781N1478 antibody
- FLJ10599 antibody
- FLJ11269 antibody
see all
Anti-Rif1 antibody images
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Western blot - Rif1 antibody (ab13422)Anti-Rif1 antibody (ab13422) at 1 µg/ml + 25 ug of MEF whole cell lysate
Secondary
IRDye TM800 conjugated goat anti rabbit IgG (H&L) incubated for 45 minutes at 1/10000 dilution
Predicted band size : 265 kDa
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Rif1 antibody (ab13422)
This product has been referenced in:
- Cooper CD et al. Identification and Characterization of Peripheral T-Cell Lymphoma-Associated SEREX Antigens. PLoS One 6:e23916 (2011). WB ; Human . Read more (PubMed: 21887344) »
- Moorhead GB et al. Displacement affinity chromatography of protein phosphatase one (PP1) complexes. BMC Biochem 9:28 (2008). WB, IP ; Human . Read more (PubMed: 19000314) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"