Overview

  • Product name
  • Description
    Rabbit polyclonal to Rif1
  • Specificity
    The immunogen sequence for ab13422 represents the C-terminal end of mouse Rif1, mouse teleomere-associated protein RIF1 and mouse Rap1-interacting factor.
  • Tested applications
    Suitable for: IP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-RNLQSRWRSPAHENS

    conjugated to KLH, corresponding to C terminal amino acids 531-545 of Mouse Rif1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
ELISA
WB
  • Application notes
    ELISA: 1/5000 - 1/40000.
    IP: Use at an assay dependent dilution (PMID 19000314).
    WB: 1/500 - 1/3000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
    • Tissue specificity
      Highly expressed in testis.
    • Developmental stage
      Expression peaks in late G2/S phase of the cell cycle.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781N1478 antibody
      • FLJ10599 antibody
      • FLJ11269 antibody
      • FLJ12870 antibody
      • Rap1 interacting factor 1 antibody
      • Rap1 interacting factor 1 homolog antibody
      • RAP1 interacting factor homolog antibody
      • RAP1 interacting factor homolog (yeast) antibody
      • Rap1-interacting factor 1 homolog antibody
      • Receptor interacting factor 1 antibody
      • RIF 1 antibody
      • RIF1 antibody
      • RIF1 isoform 4 antibody
      • RIF1_HUMAN antibody
      • Telomere associated protein RIF 1 antibody
      • Telomere associated protein RIF1 antibody
      • Telomere-associated protein RIF1 antibody
      • Telomeric protein RIF 1 antibody
      • Telomeric protein RIF1 antibody
      see all

    Images

    • Anti-Rif1 antibody (ab13422) at 1 µg/ml + 25 ug of MEF whole cell lysate

      Secondary
      IRDye TM800 conjugated goat anti rabbit IgG (H&L) incubated for 45 minutes at 1/10000 dilution

      Predicted band size : 265 kDa
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Dan J  et al. Rif1 maintains telomere length homeostasis of ESCs by mediating heterochromatin silencing. Dev Cell 29:7-19 (2014). Read more (PubMed: 24735877) »
    • Daxinger L  et al. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse. Genome Biol 14:R96 (2013). Read more (PubMed: 24025402) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cell)
    Loading amount
    20 µg
    Specification
    Mouse Embryonic Stem Cell
    Gel Running Conditions
    Reduced Denaturing (8% Bis-Tris gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 03 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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