• Product name
  • Description
    Rabbit polyclonal to Rif1
  • Host species
  • Specificity
    Reactivity with mouse Rif1 is not expected owing to divergence of sequence between this ortholog and human Rif1 at the site to which the epitope maps.
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human Rif1 (NP_060621.3)

  • Positive control
    • Whole cell lysate from 293T cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7.0-8.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab70254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 1-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
    • Tissue specificity
      Highly expressed in testis.
    • Developmental stage
      Expression peaks in late G2/S phase of the cell cycle.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781N1478 antibody
      • FLJ10599 antibody
      • FLJ11269 antibody
      • FLJ12870 antibody
      • Rap1 interacting factor 1 antibody
      • Rap1 interacting factor 1 homolog antibody
      • RAP1 interacting factor homolog antibody
      • RAP1 interacting factor homolog (yeast) antibody
      • Rap1-interacting factor 1 homolog antibody
      • Receptor interacting factor 1 antibody
      • RIF 1 antibody
      • RIF1 antibody
      • RIF1 isoform 4 antibody
      • RIF1_HUMAN antibody
      • Telomere associated protein RIF 1 antibody
      • Telomere associated protein RIF1 antibody
      • Telomere-associated protein RIF1 antibody
      • Telomeric protein RIF 1 antibody
      • Telomeric protein RIF1 antibody
      see all


    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling Rif1 with ab70254 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunoprecipitation analysis of 293T whole cell lysate (1mg for IP, 25% of reaction loaded), using ab70254 at 5 µg/mg lysate for IP. Immunoprecipitated Rif1 was blotted using ab70307 at 0.4µg/ml.


    This product has been referenced in:
    • Hiraga SI  et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep 18:403-419 (2017). IP ; Human . Read more (PubMed: 28077461) »
    • Alver RC  et al. Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1. Cell Rep 18:2508-2520 (2017). Read more (PubMed: 28273463) »

    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please find below the details about the IP experiments for ab70254:

    IP lysis Buffer (also used as wash buffer)

    12.5 ml 1M NaCl (250mM)

    2.5 ml 1M Tris (50mM)


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