Overview

  • Product nameAnti-Rif1 antibody
    See all Rif1 primary antibodies
  • Description
    Rabbit polyclonal to Rif1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 2425 and the C-terminus (residue 2472) of Human Rif1 (NP_060621.3).

  • Positive control
    • Human seminoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
    • Tissue specificityHighly expressed in testis.
    • Developmental stageExpression peaks in late G2/S phase of the cell cycle.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781N1478 antibody
      • FLJ10599 antibody
      • FLJ11269 antibody
      • FLJ12870 antibody
      • Rap1 interacting factor 1 antibody
      • Rap1 interacting factor 1 homolog antibody
      • RAP1 interacting factor homolog antibody
      • RAP1 interacting factor homolog (yeast) antibody
      • Rap1-interacting factor 1 homolog antibody
      • Receptor interacting factor 1 antibody
      • RIF 1 antibody
      • RIF1 antibody
      • RIF1 isoform 4 antibody
      • RIF1_HUMAN antibody
      • Telomere associated protein RIF 1 antibody
      • Telomere associated protein RIF1 antibody
      • Telomere-associated protein RIF1 antibody
      • Telomeric protein RIF 1 antibody
      • Telomeric protein RIF1 antibody
      see all

    Anti-Rif1 antibody images

    • ab84785, at 1/250 dilution, staining Rif1 in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB staining.

    References for Anti-Rif1 antibody (ab84785)

    ab84785 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84785.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"