The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Rap1 interacting factor 1
Rap1 interacting factor 1 homolog
RAP1 interacting factor homolog
RAP1 interacting factor homolog (yeast)
Rap1-interacting factor 1 homolog
Receptor interacting factor 1
RIF1 isoform 4
Telomere associated protein RIF 1
Telomere associated protein RIF1
Telomere-associated protein RIF1
Telomeric protein RIF 1
Telomeric protein RIF1
FunctionRequired for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
Tissue specificityHighly expressed in testis.
Developmental stageExpression peaks in late G2/S phase of the cell cycle.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.