Overview

  • Product name
  • Description
    Goat polyclonal to RIL
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide:

    DVVAVYPNAKVELV

    , corresponding to C terminal amino acids 317-330 of Human RIL.

  • Positive control
    • A431 lysates.
  • General notes
    GenBank Accession Number – NP_003678.

Properties

Applications

Our Abpromise guarantee covers the use of ab6045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
WB
  • Application notes
    IHC-FoFr: Use at an assay dependent dilution (PMID 19293990).
    WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-RIL antibody (ab6045) at 0.2 µg/ml + Lysate prepared from A431 cells. (RIPA buffer, 35µg total protein per lane).

      Developed using the ECL technique.

      Predicted band size: 35 kDa


      Exposure time: 1 hour


      ab6045 at 0.2µg/ml staining approximately 37kDa band of RIL in A431 Lysate by Western blot (ECL).

    References

    This product has been referenced in:
    • Miyazaki K  et al. CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Exp Cell Res 318:1716-25 (2012). WB, ICC/IF ; Rat . Read more (PubMed: 22659164) »
    • Guryanova OA  et al. Actin Cytoskeleton Remodeling by the Alternatively Spliced Isoform of PDLIM4/RIL Protein. J Biol Chem 286:26849-59 (2011). WB . Read more (PubMed: 21636573) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab6045.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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