The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-FoFr: Use at an assay dependent dilution (PMID 19293990).
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Anti-RIL antibody (ab6045) at 0.2 µg/ml + Lysate prepared from A431 cells. (RIPA buffer, 35µg total protein per lane). Developed using the ECL technique
Predicted band size : 35 kDa
Exposure time : 1 hour
References for Anti-RIL antibody (ab6045)
This product has been referenced in:
Miyazaki K et al. CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Exp Cell Res318:1716-25 (2012).
Read more (PubMed: 22659164) »
Guryanova OA et al. Actin Cytoskeleton Remodeling by the Alternatively Spliced Isoform of PDLIM4/RIL Protein. J Biol Chem286:26849-59 (2011).
Read more (PubMed: 21636573) »