Overview

  • Product name
  • Description
    Rabbit polyclonal to RIM2
  • Specificity
    RIM2 is known to exist in multiple isoforms; this antibody should recognize most of them. The antibody is predicted to have no cross reactivity to other RIM proteins.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: IHC-FoFr
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid synthetic peptide near the center of human RIM2

  • Positive control
    • Rat brain tissue lysate, Human brain tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 160 kDa).
  • Application notes
    Is unsuitable for IHC-FoFr.
  • Target

    • Function
      Rab effector involved in exocytosis. May act as scaffold protein.
    • Sequence similarities
      Contains 2 C2 domains.
      Contains 1 FYVE-type zinc finger.
      Contains 1 PDZ (DHR) domain.
      Contains 1 RabBD (Rab-binding) domain.
    • Cellular localization
      Cell membrane. Cell junction > synapse. Cell junction > synapse > presynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0751 antibody
      • Non small cell lung cancer RimL3a protein antibody
      • Non small cell lung cancer RimL3c protein antibody
      • OBOE antibody
      • Protein regulating synaptic membrane exocytosis 2 antibody
      • Rab-3-interacting molecule 2 antibody
      • Rab-3-interacting protein 3 antibody
      • Rab3 interacting molecule 2 antibody
      • RAB3IP3 antibody
      • Regulating synaptic membrane exocytosis 2 antibody
      • Regulating synaptic membrane exocytosis protein 2 antibody
      • RIM 2 antibody
      • Rims2 (gene name) antibody
      • Rims2 antibody
      • RIMS2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RIM2 antibody (ab69860) at 1 µg/ml

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat brain tissue lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 160 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Note: There are seven named isoforms of this protein.
    • ab69860 at 5 µg/ml staining RIM2 in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

    References

    This product has been referenced in:
    • Park JH  et al. Glucagon-like peptide-1 enhances glucokinase activity in pancreatic ß-cells through the association of Epac2 with Rim2 and Rab3A. Endocrinology 153:574-82 (2012). WB ; Human . Read more (PubMed: 22147008) »

    See 1 Publication for this product

    Customer reviews and Q&As



    As we discussed, I'd recommend pre-clearing the lysates with normal rabbit serum to remove anything in the lysate that binds non-specifically with IgG. You can also look through the literature to see if other researchers have reported an isof...

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    Thank you for your inquiry.

    I have to confirm that the exact immunogen sequence of ab69860is proprietary information, but it is from within amino acids 201-260 of human RIM2.

    Therefore this antibody should be suitable for a recombin...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Cortex)
    Specification
    Cortex
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Dr. Karine Thibault

    Verified customer

    Submitted Nov 08 2010

    Thank you for your enquiry. This product detects isoform 4, which is predicted to be about 91.9kDa in size. Based on the immunogen sequence, this antibody will cross-react with human isoforms 2 through 6 of human RIM2, and mice and rats should h...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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