Overview

  • Product nameAnti-RIM2 antibody
    See all RIM2 primary antibodies
  • Description
    Rabbit polyclonal to RIM2
  • SpecificityRIM2 is known to exist in multiple isoforms; this antibody should recognize most of them. The antibody is predicted to have no cross reactivity to other RIM proteins.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: IHC-FoFr
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid synthetic peptide near the center of human RIM2

  • Positive control
    • Rat brain tissue lysate, Human brain tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 160 kDa).
  • Application notesIs unsuitable for IHC-FoFr.
  • Target

    • FunctionRab effector involved in exocytosis. May act as scaffold protein.
    • Sequence similaritiesContains 2 C2 domains.
      Contains 1 FYVE-type zinc finger.
      Contains 1 PDZ (DHR) domain.
      Contains 1 RabBD (Rab-binding) domain.
    • Cellular localizationCell membrane. Cell junction > synapse. Cell junction > synapse > presynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0751 antibody
      • Non small cell lung cancer RimL3a protein antibody
      • Non small cell lung cancer RimL3c protein antibody
      • OBOE antibody
      • Protein regulating synaptic membrane exocytosis 2 antibody
      • Rab-3-interacting molecule 2 antibody
      • Rab-3-interacting protein 3 antibody
      • Rab3 interacting molecule 2 antibody
      • RAB3IP3 antibody
      • Regulating synaptic membrane exocytosis 2 antibody
      • Regulating synaptic membrane exocytosis protein 2 antibody
      • RIM 2 antibody
      • Rims2 (gene name) antibody
      • Rims2 antibody
      • RIMS2_HUMAN antibody
      see all

    Anti-RIM2 antibody images

    • All lanes : Anti-RIM2 antibody (ab69860) at 1 µg/ml

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat brain tissue lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 160 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Note: There are seven named isoforms of this protein.
    • ab69860 at 5 µg/ml staining RIM2 in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

    References for Anti-RIM2 antibody (ab69860)

    This product has been referenced in:
    • Park JH  et al. Glucagon-like peptide-1 enhances glucokinase activity in pancreatic ß-cells through the association of Epac2 with Rim2 and Rab3A. Endocrinology 153:574-82 (2012). WB ; Human . Read more (PubMed: 22147008) »

    See 1 Publication for this product

    Product Wall



    As we discussed, I'd recommend pre-clearing the lysates with normal rabbit serum to remove anything in the lysate that binds non-specifically with IgG. You can also look through the literature to see if other researchers have reported an isof...

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    Thank you for your inquiry.

    I have to confirm that the exact immunogen sequence of ab69860is proprietary information, but it is from within amino acids 201-260 of human RIM2.

    Therefore this antibody should be suitable for a recombin...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Cortex)
    Specification Cortex
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Karine Thibault

    Verified customer

    Submitted Nov 08 2010

    Thank you for your enquiry. This product detects isoform 4, which is predicted to be about 91.9kDa in size. Based on the immunogen sequence, this antibody will cross-react with human isoforms 2 through 6 of human RIM2, and mice and rats should h...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"