Overview

  • Product nameAnti-RIN1 antibody
    See all RIN1 primary antibodies
  • Description
    Mouse polyclonal to RIN1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    RIN1 human protein (NP_004283).

  • Positive control
    • RIN1 transfected 293T cell lysate, rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab69897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 87 and 100 kDa in two separate blots (predicted molecular weight: 84 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceRIN1 is a Ras effector protein which can affect Ras signaling in a variety of ways. It enhances signaling from ABL1 and ABL2 whcih are involved in regulation of cytoskeletal remodeling. It may function as a guanine nucleotide exchange factor in activation of Rab5a. Rin1 also competes with RAF1 for binding of the activated form of Ras. It may play a role inhibiting the modulation of neuronal plasticity in formation of aversive memory, and has been implicated as a tumor supressor gene in breast cancer.
    • Cellular localizationCytoplasmic and Plasma membrane
    • Database links
    • Alternative names
      • Ras and Rab interactor 1 antibody
      • ras inhibitor 1 antibody
      • ras inhibitor antibody
      • Ras inhibitor JC99 antibody
      • ras inhibitor RIN1 antibody
      • Ras interaction/interference protein 1 antibody
      • RIN 1 antibody
      see all

    Anti-RIN1 antibody images

    • Anti-RIN1 antibody (ab69897) at 1/500 dilution + rat brain at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 84 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-RIN1 antibody (ab69897) at 1/500 dilution

      Lane 1 : RIN1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 84 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    References for Anti-RIN1 antibody (ab69897)

    This product has been referenced in:
    • Kiel C  et al. Integration of protein abundance and structure data reveals competition in the ErbB signaling network. Sci Signal 6:ra109 (2013). Read more (PubMed: 24345680) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab69897.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"