Anti-RING finger protein unkempt like antibody (ab68731)

Overview

  • Product nameAnti-RING finger protein unkempt like antibody
    See all RING finger protein unkempt like primary antibodies
  • Description
    Mouse polyclonal to RING finger protein unkempt like
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    RING finger protein unkempt like (NP_001032202, 1 a.a. ~ 277 a.a) full-length human protein.

  • Positive control
    • RING finger protein unkempt like transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).

Target

  • FunctionMay participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the unkempt family.
    Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • DomainAlthough this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946).
  • Post-translational
    modifications
    Isoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur.
  • Cellular localizationCytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • C16orf28 antibody
    • FLJ12623 antibody
    • FLJ23360 antibody
    • KIAA0734 antibody
    • MGC5179 antibody
    • Putative E3 ubiquitin-protein ligase UNKL antibody
    • RING finger protein unkempt-like antibody
    • Unkempt homolog (Drosophila) like antibody
    • Unkempt like antibody
    • Unkempt like protein antibody
    • Unkl antibody
    • UNKL_HUMAN antibody
    • ZC3H5L antibody
    • ZC3HDC5L antibody
    • Zinc finger CCCH domain-containing protein 5-like antibody
    see all

Anti-RING finger protein unkempt like antibody images

  • All lanes : Anti-RING finger protein unkempt like antibody (ab68731) at 1/500 dilution

    Lane 1 : RING finger protein unkempt like transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-RING finger protein unkempt like antibody (ab68731)

ab68731 has not yet been referenced specifically in any publications.

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