The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).
FunctionMay participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the unkempt family. Contains 4 C3H1-type zinc fingers. Contains 1 RING-type zinc finger.
DomainAlthough this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946).
Post-translational modificationsIsoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur.
Cellular localizationCytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm.