Anti-RING finger protein unkempt like antibody - N-terminal (ab155197)

Overview

  • Product name
    Anti-RING finger protein unkempt like antibody - N-terminal
  • Description
    Rabbit polyclonal to RING finger protein unkempt like - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-222 of Human RING finger protein unkempt like (Uniprot ID: Q9H9P5).

  • Positive control
    • HeLa whole cell lysate; HeLa cells
  • General notes
    Keep as a concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 81,75, 32, 25 kDa.

Target

  • Function
    May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the unkempt family.
    Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • Domain
    Although this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946).
  • Post-translational
    modifications
    Isoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur.
  • Cellular localization
    Cytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • C16orf28 antibody
    • FLJ12623 antibody
    • FLJ23360 antibody
    • KIAA0734 antibody
    • MGC5179 antibody
    • Putative E3 ubiquitin-protein ligase UNKL antibody
    • RING finger protein unkempt-like antibody
    • Unkempt homolog (Drosophila) like antibody
    • Unkempt like antibody
    • Unkempt like protein antibody
    • Unkl antibody
    • UNKL_HUMAN antibody
    • ZC3H5L antibody
    • ZC3HDC5L antibody
    • Zinc finger CCCH domain-containing protein 5-like antibody
    see all

Images

  • Anti-RING finger protein unkempt like antibody - N-terminal (ab155197) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 81,75, 32, 25 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling RING finger protein unkempt like with ab155197 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Avet-Rochex A  et al. Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control. PLoS Genet 10:e1004624 (2014). Read more (PubMed: 25210733) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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