Overview

  • Product name
  • Description
    Rabbit polyclonal to RING1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Monkey
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 31 and 170 of Human RING1 (NP_002922).

  • Positive control
    • 293T whole cell lysate; PC9 xenograft tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (PH8.0).

Target

  • Function
    Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ring1A antibody
    • E3 ubiquitin-protein ligase RING1 antibody
    • Polycomb complex protein RING1 antibody
    • Really interesting new gene 1 protein antibody
    • RING finger protein 1 antibody
    • Ring1 antibody
    • RING1_HUMAN antibody
    • RING1A antibody
    • Rnf1 antibody
    • Transcription repressor Ring1A antibody
    see all

Images

  • Anti-RING1 antibody (ab102469) at 1/3000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 42 kDa
  • ab102469, at a 1/500 dilution, staining RING1 in paraffin embedded PC9 xenograft by Immunohistochemistry.

References

ab102469 has not yet been referenced specifically in any publications.

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