Overview

  • Product nameAnti-RING1 antibody - ChIP Grade
    See all RING1 primary antibodies
  • Description
    Rabbit polyclonal to RING1 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human RING1.

    (Peptide available as ab32805.)

  • Positive control
    • This antibody gave a positive control in the following human lysates: Kidney tissue Liver tissue This antibody gave a positive control in the following mouse lysate: Kidney tissue This antibody gave a positive control in the following rat lysate: Kidney Whole Cell Lysate - normal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab32644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 20237320
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42,39 kDa).

Target

  • FunctionConstitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Cellular localizationNucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ring1A antibody
    • E3 ubiquitin-protein ligase RING1 antibody
    • Polycomb complex protein RING1 antibody
    • Really interesting new gene 1 protein antibody
    • RING finger protein 1 antibody
    • Ring1 antibody
    • RING1_HUMAN antibody
    • RING1A antibody
    • Rnf1 antibody
    • Transcription repressor Ring1A antibody
    see all

Anti-RING1 antibody - ChIP Grade images

  • All lanes : Anti-RING1 antibody - ChIP Grade (ab32644) at 1 µg/ml

    Lane 1 : Human Kidney Whole Tissue Lysate
    Lane 2 : Human Liver Whole Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 42,39 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RING1 antibody - ChIP Grade (ab32644) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42,39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-RING1 antibody - ChIP Grade (ab32644)

This product has been referenced in:
  • Basu A  et al. YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment. Nucleic Acids Res N/A:N/A (2013). ChIP ; Human . Read more (PubMed: 24285299) »
  • Argiropoulos B  et al. Linkage of the potent leukemogenic activity of Meis1 to cell-cycle entry and transcriptional regulation of cyclin D3. Blood 115:4071-82 (2010). ChIP ; Mouse . Read more (PubMed: 20237320) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"