The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
PathwayProtein modification; protein ubiquitination.
Post-translational modificationsPolyubiquitinated in the presence of UBE2D3 (in vitro). Monoubiquitinated, by auto-ubiquitination.
Cellular localizationNucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
Huntingtin interacting protein 2 interacting protein 3 antibody
Huntingtin-interacting protein 2-interacting protein 3 antibody
Polycomb M33 interacting protein Ring 1B antibody
Polycomb M33 interacting protein Ring1B antibody
Protein DinG antibody
RING 1B antibody
RING 2 antibody
RING finger protein 1B antibody
RING finger protein 2 antibody
RING finger protein BAP 1 antibody
RING finger protein BAP-1 antibody
RING finger protein BAP1 antibody
RNF 2 antibody
Anti-RING2 / RING1B / RNF2 antibody images
Western blot - RING2 / RING1B / RNF2 antibody (ab14751)
Anti-RING2 / RING1B / RNF2 antibody (ab14751) at 0.5 µg/ml + Cell lysate prepared from human K562 cells at 35 µg Developed using the ECL technique
Predicted band size : 38 kDa
References for Anti-RING2 / RING1B / RNF2 antibody (ab14751)
This product has been referenced in:
Zhang Y et al. Ubiquitination and degradation of ribonucleotide reductase M1 by the polycomb group proteins RNF2 and Bmi1 and cellular response to gemcitabine. PLoS One9:e91186 (2014).
Read more (PubMed: 24614341) »
Charan RA et al. Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin. Cell Death Dis5:e1313 (2014).
Read more (PubMed: 24991765) »