Anti-RING2 / RING1B / RNF2 antibody (ab28629)

Overview

  • Product nameAnti-RING2 / RING1B / RNF2 antibody
    See all RING2 / RING1B / RNF2 primary antibodies
  • Description
    Rabbit polyclonal to RING2 / RING1B / RNF2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide derived from residues 156 - 204 of human RNF2.

    .

  • Positive control
    • IHCP: human kidney. WB: HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Polyubiquitinated in the presence of UBE2D3 (in vitro).
    Monoubiquitinated, by auto-ubiquitination.
  • Cellular localizationNucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAP 1 antibody
    • BAP1 antibody
    • DING antibody
    • DinG protein antibody
    • E3 ubiquitin protein ligase RING 2 antibody
    • E3 ubiquitin protein ligase RING2 antibody
    • E3 ubiquitin-protein ligase RING2 antibody
    • HIP2 interacting protein 3 antibody
    • HIP2-interacting protein 3 antibody
    • HIPI 3 antibody
    • HIPI3 antibody
    • Huntingtin interacting protein 2 interacting protein 3 antibody
    • Huntingtin-interacting protein 2-interacting protein 3 antibody
    • OTTHUMP00000060668 antibody
    • Polycomb M33 interacting protein Ring 1B antibody
    • Polycomb M33 interacting protein Ring1B antibody
    • Protein DinG antibody
    • RING 1B antibody
    • RING 2 antibody
    • RING finger protein 1B antibody
    • RING finger protein 2 antibody
    • RING finger protein BAP 1 antibody
    • RING finger protein BAP-1 antibody
    • RING finger protein BAP1 antibody
    • RING1b antibody
    • RING2_HUMAN antibody
    • RNF 2 antibody
    • Rnf2 antibody
    see all

Anti-RING2 / RING1B / RNF2 antibody images

  • Anti-RING2 / RING1B / RNF2 antibody (ab28629) at 1.25 µg/ml + HepG2 Cell Lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling RING2 / RING1B / RNF2 with ab28629 at 4-8µg/ml.Arrows point to positively labelled epithelial cells of renal tubule. Magnification: x400.

References for Anti-RING2 / RING1B / RNF2 antibody (ab28629)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.15% · Temperature: RT°C
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Verified customer

Submitted Aug 08 2012

Thank you very much for your call today. I am sorry to hear about the poor results with this antibody, but after submitting a review of ab28629 with mouse samples, you can use the discount code below to receive one free primary antibody (such as ab1155...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"