RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.
Our Abpromise guarantee covers the use of ab3832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/300. (see Abreviews)|
|WB||Use at an assay dependent dilution. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa).|
|ChIP||Use at an assay dependent dilution.|
Western blot using ab3832 at 1/500. Lysates at 20
Lane 1: 3T3 cell lysate
Lane 2: U937 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: CHO-K1 cell lysate
Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000
Exposure time: 3 minutes