Anti-RING2 / RING1B / RNF2 antibody - ChIP Grade (ab3832)

Overview

  • Product nameAnti-RING2 / RING1B / RNF2 antibody - ChIP Grade
    See all RING2 / RING1B / RNF2 primary antibodies
  • Description
    Goat polyclonal to RING2 / RING1B / RNF2 - ChIP Grade
  • SpecificityThis antibody recognises a band at just above the expected size of 37kD.
  • Tested applicationsSuitable for: ICC/IF, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    EAGPSNKRTKTSDC

    , corresponding to amino acids 189-201 of Human RING1B.

  • General notes

    RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesRING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300. (see Abreviews)
WB Use at an assay dependent dilution. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa).
ChIP Use at an assay dependent dilution.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Polyubiquitinated in the presence of UBE2D3 (in vitro).
    Monoubiquitinated, by auto-ubiquitination.
  • Cellular localizationNucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAP 1 antibody
    • BAP1 antibody
    • DING antibody
    • DinG protein antibody
    • E3 ubiquitin protein ligase RING 2 antibody
    • E3 ubiquitin protein ligase RING2 antibody
    • E3 ubiquitin-protein ligase RING2 antibody
    • HIP2 interacting protein 3 antibody
    • HIP2-interacting protein 3 antibody
    • HIPI 3 antibody
    • HIPI3 antibody
    • Huntingtin interacting protein 2 interacting protein 3 antibody
    • Huntingtin-interacting protein 2-interacting protein 3 antibody
    • OTTHUMP00000060668 antibody
    • Polycomb M33 interacting protein Ring 1B antibody
    • Polycomb M33 interacting protein Ring1B antibody
    • Protein DinG antibody
    • RING 1B antibody
    • RING 2 antibody
    • RING finger protein 1B antibody
    • RING finger protein 2 antibody
    • RING finger protein BAP 1 antibody
    • RING finger protein BAP-1 antibody
    • RING finger protein BAP1 antibody
    • RING1b antibody
    • RING2_HUMAN antibody
    • RNF 2 antibody
    • Rnf2 antibody
    see all

Anti-RING2 / RING1B / RNF2 antibody - ChIP Grade images



  • Predicted band size : 37.6 kDa


    Western blot using ab3832 at 1/500. Lysates at 20µg/lane.

    Lane 2: U937 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: CHO-K1 cell lysate

    Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000 Exposure time: 3 minutes
  • ab3832 staining RING2/RING1B/RNF2 in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-RING2 / RING1B / RNF2 antibody - ChIP Grade (ab3832)

This product has been referenced in:
  • Wen W  et al. Knockdown of RNF2 induces apoptosis by regulating MDM2 and p53 stability. Oncogene : (2013). ChIP . Read more (PubMed: 23318437) »
  • Cuddapah S  et al. A novel human polycomb binding site acts as a functional polycomb response element in Drosophila. PLoS One 7:e36365 (2012). ChIP ; Human . Read more (PubMed: 22570707) »

See all 7 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Specification Hela cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
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Verified customer

Submitted Aug 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 100000 cells
Specification Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 17 2010

Thank you for your enquiry. I have consulted the EXPASY databases and I retrieved the following information: Mel18 - Tissue specificity: Detected in all tissues examined with high expression found in placenta, lung and kidney and low expression, in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"