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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline, pH 7-8
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
Use at 2-5 µg/mg of lysate.
Sequence similarities Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. Contains 1 protein kinase domain.
Information by UniProt
RIO kinase 1 (yeast) antibody
Anti-RIOK1 antibody images
Western blot - Anti-RIOK1 antibody (ab88496)
All lanes : Anti-RIOK1 antibody (ab88496) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size : 66 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-RIOK1 antibody (ab88496)
3ug ab88496 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88496 at 0.4ug/ml.
Detection: chemoluminescence with exposure time of 30 seconds.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"