Overview

  • Product name
  • Description
    Rabbit polyclonal to RIOK1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide mapping to a region between residue 1 and 50 of human RIOK1 using the numbering given in entry (NP_113668.2)

  • Positive control
    • Whole cell lysate from HeLa or 293T cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Sequence similarities
    Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD034 antibody
    • bA288G3.1 antibody
    • RIO kinase 1 (yeast) antibody
    • RIO kinase 1 antibody
    • RIOK1 antibody
    • RIOK1_HUMAN antibody
    • Serine/threonine-protein kinase rio1 antibody
    see all

Images

  • All lanes : Anti-RIOK1 antibody (ab88496) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 66 kDa


    Exposure time : 3 minutes
  • 3ug ab88496 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88496 at 0.4ug/ml.
    Detection: chemoluminescence with exposure time of 30 seconds.

References

This product has been referenced in:
  • Weinberg F  et al. The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior. EBioMedicine N/A:N/A (2017). WB ; Human . Read more (PubMed: 28499923) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (epithelial)
Permeabilization
No
Specification
epithelial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Methanol
Username

De Wulf Lab CIBIO

Verified customer

Submitted Nov 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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