Overview

  • Product nameAnti-RIP antibody [EPR4689]
    See all RIP primary antibodies
  • Description
    Rabbit monoclonal [EPR4689] to RIP
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RIP aa 300-450 (internal sequence).

  • Positive control
    • Raji, Jurkat, HeLa and 293T cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab125072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionEssential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
      Autophosphorylated on serine and threonine residues.
      Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell death protein RIP antibody
      • FLJ39204 antibody
      • OTTHUMP00000039163 antibody
      • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
      • receptor interacting protein 1 antibody
      • Receptor interacting protein antibody
      • Receptor interacting protein kinase 1 antibody
      • Receptor interacting serine threonine protein kinase 1 antibody
      • Receptor TNFRSF interacting serine threonine kinase 1 antibody
      • Receptor-interacting protein 1 antibody
      • Receptor-interacting serine/threonine-protein kinase 1 antibody
      • Rinp antibody
      • RIP 1 antibody
      • RIP antibody
      • Rip-1 antibody
      • RIP1 antibody
      • RIPK 1 antibody
      • Ripk1 antibody
      • RIPK1_HUMAN antibody
      • Serine threonine protein kinase RIP antibody
      • Serine/threonine-protein kinase RIP antibody
      see all

    Anti-RIP antibody [EPR4689] images



    • Predicted band size : 75 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: RIP knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Raji cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab125072 observed at 78 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab125072 was shown to specifically react with RIP when RIP knockout samples were used. Wild-type and RIP knockout samples were subjected to SDS-PAGE. ab125072 at a dilution of 1/1000 and ab8245 (loading control to GAPDH)  at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-RIP antibody [EPR4689] (ab125072) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 75 kDa

    References for Anti-RIP antibody [EPR4689] (ab125072)

    ab125072 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"