Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesSteroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor Interacting Protein 140 (RIP 140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP 140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab3425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 129 kDa).Can be blocked with RIP140 peptide (ab4968).

Target

  • FunctionModulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
  • DomainContains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
  • Post-translational
    modifications
    Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
  • Cellular localizationNucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
  • Information by UniProt
  • Database links
  • Alternative names
    • NRIP 1 antibody
    • NRIP1 antibody
    • NRIP1_HUMAN antibody
    • Nuclear factor RIP 140 antibody
    • Nuclear factor RIP140 antibody
    • Nuclear receptor interacting protein 1 antibody
    • Nuclear receptor-interacting protein 1 antibody
    • Receptor interacting protein 140 antibody
    • Receptor-interacting protein 140 antibody
    • RIP 140 antibody
    • RIP140 antibody
    see all

Anti-RIP140 antibody images



  • Predicted band size : 129 kDa

    Western blot of recombinant Human RIP140 using ab3425.

     

References for Anti-RIP140 antibody (ab3425)

This product has been referenced in:
  • Al-Rewashdy H  et al. Utrophin A is essential in mediating the functional adaptations of mdx mouse muscle following chronic AMPK activation. Hum Mol Genet 24:1243-55 (2015). Read more (PubMed: 25324540) »
  • Vanhorebeek I  et al. Mitochondrial fusion, fission, and biogenesis in prolonged critically ill patients. J Clin Endocrinol Metab 97:E59-64 (2012). WB ; Human . Read more (PubMed: 22013100) »

See all 8 Publications for this product

Product Wall

Yes, the immunogen was derived from that sequence. It is in figure 1.a. of the 1995 reference:

http://www.ncbi.nlm.nih.gov/pubmed/7641693

Apparently the sequence data was modified over time to yield the current sequence at the Uni...

Read More

The percentage identity depends on which sequence you align with the immunogen. Aligning with the sequence at the UniProt entry, http://www.uniprot.org/uniprot/P48552 returns 78%. BLAST the immunogen and the top hit is CAA59108.1, which aligns 100%. Read More

Gracias por tu respuesta.

El anticuerpo ab12193 está en stock, y si el pedido se tramita en el dia de hoy, os llegaría mañana mismo.

Antes de hacerlo, me gustaría informarte de que actualmente tenemos una...

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Thank you for your inquiry and I sincerely apologize for the long delay. We had to wait on the supplying lab's Marketing Manager to look back through the historic information for this antibody. The sequence that is listed on our datasheet is correc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"