Anti-RIP140 antibody (ab42126)
Key features and details
- Rabbit polyclonal to RIP140
- Suitable for: IP, WB, IHC-Fr, IHC (PFA fixed), ChIP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RIP140 antibody
See all RIP140 primary antibodies -
Description
Rabbit polyclonal to RIP140 -
Host species
Rabbit -
Specificity
ab42126 recognises the C terminal domain of RIP140 -
Tested applications
Suitable for: IP, WB, IHC-Fr, IHC (PFA fixed), ChIP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (Human) corresponding to the C terminal domain of RIP140. Peptide available as ab93493.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), 69.5% Tris HCl -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC (PFA fixed) |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration. PubMed: 21360626
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IHC-P | (1) |
Use a concentration of 4 µg/ml.
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC (PFA fixed)
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 21360626 |
IHC-P
Use a concentration of 4 µg/ml. |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. -
Domain
Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding. -
Post-translational
modificationsAcetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH. -
Cellular localization
Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1. - Information by UniProt
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Database links
- Entrez Gene: 8204 Human
- Entrez Gene: 268903 Mouse
- Entrez Gene: 304157 Rat
- Omim: 602490 Human
- SwissProt: P48552 Human
- SwissProt: Q8CBD1 Mouse
- Unigene: 155017 Human
- Unigene: 455873 Mouse
see all -
Alternative names
- NRIP 1 antibody
- NRIP1 antibody
- NRIP1_HUMAN antibody
see all
Images
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ab42126 (4µg/ml) staining RIP140 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the nuclei of the intestinal glands.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
ICC/IF image of ab42126 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (35)
ab42126 has been referenced in 35 publications.
- Jacquier V et al. RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53. Cell Mol Life Sci 79:270 (2022). PubMed: 35501580
- Lin YW et al. Regulation of exosome secretion by cellular retinoic acid binding protein 1 contributes to systemic anti-inflammation. Cell Commun Signal 19:69 (2021). PubMed: 34193153
- Gleizes A et al. RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34206767
- Palassin P et al. A Truncated NRIP1 Mutant Amplifies Microsatellite Instability of Colorectal Cancer by Regulating MSH2/MSH6 Expression, and Is a Prognostic Marker of Stage III Tumors. Cancers (Basel) 13:N/A (2021). PubMed: 34503257
- Tsagkaraki E et al. CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease. Nat Commun 12:6931 (2021). PubMed: 34836963