Overview

  • Product name
  • Description
    Goat polyclonal to RIP140
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-EKDSPRLTKTNP

    , corresponding to internal sequence amino acids 1055-1066 of Human RIP140 (NP_003480.2).

  • Positive control
    • Human Cerebellum lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 129 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
    • Domain
      Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
    • Post-translational
      modifications
      Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
    • Cellular localization
      Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
    • Information by UniProt
    • Database links
    • Alternative names
      • NRIP 1 antibody
      • NRIP1 antibody
      • NRIP1_HUMAN antibody
      • Nuclear factor RIP 140 antibody
      • Nuclear factor RIP140 antibody
      • Nuclear receptor interacting protein 1 antibody
      • Nuclear receptor-interacting protein 1 antibody
      • Receptor interacting protein 140 antibody
      • Receptor-interacting protein 140 antibody
      • RIP 140 antibody
      • RIP140 antibody
      see all

    Images

    • Anti-RIP140 antibody (ab91476) at 0.5 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 129 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    References

    ab91476 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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