Anti-RIP140 antibody - Aminoterminal end (ab42125)

Overview

  • Product nameAnti-RIP140 antibody - Aminoterminal end
    See all RIP140 primary antibodies
  • Description
    Rabbit polyclonal to RIP140 - Aminoterminal end
  • Specificityab42125 recognises RIP140 at the N terminal region containing the HDAC binding domain.
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to the N terminal domain of RIP140.

Properties

Applications

Our Abpromise guarantee covers the use of ab42125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notesIP: 1/250. Note: ab42125 can be used for IP pull-down assays.
    WB: 1/1000. Predicted molecular weight: 129 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionModulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
    • DomainContains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
    • Post-translational
      modifications
      Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
    • Cellular localizationNucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
    • Information by UniProt
    • Database links
    • Alternative names
      • NRIP 1 antibody
      • NRIP1 antibody
      • NRIP1_HUMAN antibody
      • Nuclear factor RIP 140 antibody
      • Nuclear factor RIP140 antibody
      • Nuclear receptor interacting protein 1 antibody
      • Nuclear receptor-interacting protein 1 antibody
      • Receptor interacting protein 140 antibody
      • Receptor-interacting protein 140 antibody
      • RIP 140 antibody
      • RIP140 antibody
      see all

    References for Anti-RIP140 antibody - Aminoterminal end (ab42125)

    ab42125 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (MCF7)
    Loading amount 50 µg
    Specification MCF7
    Gel Running Conditions Non-reduced Denaturing (8% Acrylamide)
    Blocking step Milk as blocking agent for 25 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 01 2011

    Application Western blot
    Sample Monkey Cell lysate - nuclear (COS7)
    Loading amount 50 µg
    Specification COS7
    Gel Running Conditions Non-reduced Denaturing (6% Acrylamide)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 30 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"