Anti-RIP140 antibody - ChIP Grade (ab42126)

Overview

  • Product nameAnti-RIP140 antibody - ChIP Grade
    See all RIP140 primary antibodies
  • Description
    Rabbit polyclonal to RIP140 - ChIP Grade
  • Specificityab42126 recognises the C terminal domain of RIP140
  • Tested applicationsSuitable for: IHC-P, IHC (PFA fixed), IHC-Fr, IP, WB, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to the C terminal domain of RIP140. Peptide available as ab93493.

Properties

Applications

Our Abpromise guarantee covers the use of ab42126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml.
IHC (PFA fixed) Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP 1/250.
WB 1/1000. Predicted molecular weight: 140 kDa.
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 21360626

Target

  • FunctionModulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
  • DomainContains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
  • Post-translational
    modifications
    Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
  • Cellular localizationNucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
  • Information by UniProt
  • Database links
  • Alternative names
    • NRIP 1 antibody
    • NRIP1 antibody
    • NRIP1_HUMAN antibody
    • Nuclear factor RIP 140 antibody
    • Nuclear factor RIP140 antibody
    • Nuclear receptor interacting protein 1 antibody
    • Nuclear receptor-interacting protein 1 antibody
    • Receptor interacting protein 140 antibody
    • Receptor-interacting protein 140 antibody
    • RIP 140 antibody
    • RIP140 antibody
    see all

Anti-RIP140 antibody - ChIP Grade images

  • ab42126 (4µg/ml) staining RIP140 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the nuclei of the intestinal glands.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab42126 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RIP140 antibody - ChIP Grade (ab42126)

This product has been referenced in:
  • Lin YW  et al. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages. J Mol Cell Cardiol 79:287-94 (2015). WB, IHC-Fr, ChIP ; Mouse . Read more (PubMed: 25528964) »
  • Wu CY  et al. Coordinated repressive chromatin-remodeling of Oct4 and Nanog genes in RA-induced differentiation of embryonic stem cells involves RIP140. Nucleic Acids Res 42:4306-17 (2014). ChIP ; Mouse . Read more (PubMed: 24489122) »

See all 14 Publications for this product

Product Wall

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I can confirm, that to our knowledge, customised protocols are not required for the use of ab42126 - Anti-RIP140 antibody - ChIP Grade in IP. However, I can recommend to have a look at our standard protocol, provid...

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Thank you for your inquiry and your patience with this reply. I apologize that it took the testing laboratory a while to confirm how this antibody has been further tested. The lab confirmed that the antibody has been tested for labeling endogenou...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 18 2014

Thank you for contacting us. This antibody was generate from an 18 aa peptide from the C terminal.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

This product's country of origin is the United States.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"