Anti-RIP140 antibody - ChIP Grade (ab42126)

Abreviews (1)Q&A (5)Specific References (19)

Overview

  • Product name
    Anti-RIP140 antibody - ChIP Grade
    See all RIP140 primary antibodies
  • Description
    Rabbit polyclonal to RIP140 - ChIP Grade
  • Host species
    Rabbit
  • Specificity
    ab42126 recognises the C terminal domain of RIP140
  • Tested applications
    Suitable for: IHC-P, IHC (PFA fixed), IHC-Fr, IP, WB, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to the C terminal domain of RIP140. Peptide available as ab93493.

Properties

Applications

Our Abpromise guarantee covers the use of ab42126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml.
IHC (PFA fixed) Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP 1/250.
WB 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 21360626

Target

  • Function
    Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
  • Domain
    Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
  • Post-translational
    modifications
    Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
  • Cellular localization
    Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • NRIP 1 antibody
    • NRIP1 antibody
    • NRIP1_HUMAN antibody
    • Nuclear factor RIP 140 antibody
    • Nuclear factor RIP140 antibody
    • Nuclear receptor interacting protein 1 antibody
    • Nuclear receptor-interacting protein 1 antibody
    • Receptor interacting protein 140 antibody
    • Receptor-interacting protein 140 antibody
    • RIP 140 antibody
    • RIP140 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP140 antibody - ChIP Grade (ab42126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP140 antibody - ChIP Grade (ab42126)
    ab42126 (4µg/ml) staining RIP140 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the nuclei of the intestinal glands.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-RIP140 antibody - ChIP Grade (ab42126)
    Immunocytochemistry/ Immunofluorescence - Anti-RIP140 antibody - ChIP Grade (ab42126)
    ICC/IF image of ab42126 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Lin YW  et al. Glyburide and retinoic acid synergize to promote wound healing by anti-inflammation and RIP140 degradation. Sci Rep 8:834 (2018). Read more (PubMed: 29339732) »
  • Lin YW  et al. Receptor-Interacting Protein 140 Orchestrates the Dynamics of Macrophage M1/M2 Polarization. J Innate Immun 8:97-107 (2016). Read more (PubMed: 26228026) »

See all 19 Publications for this product

Publishing research using ab42126? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

Inquiry: Dear Sir or Madam, I would like to perform a co-IP using the RIP140 antibody #42126. I was thinking using RIPA buffer as a lysis buffer given that RIP140 is a nuclear protein. However, as this antibody has been validated for IP, I was wonderin...

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Thank you for contacting us.

I can confirm, that to our knowledge, customised protocols are not required for the use of ab42126 - Anti-RIP140 antibody - ChIP Grade in IP. However, I can recommend to have a look at our standard protocol, provid...

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I'm using this antibody to detect RIP140 in HeLa nuclear extracts and not seeing any signal. What was the protocol tested?

Thank you for your inquiry and your patience with this reply. I apologize that it took the testing laboratory a while to confirm how this antibody has been further tested. The lab confirmed that the antibody has been tested for labeling endogenou...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RIP140 antibody - ChIP Grade

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Apr 18 2014

WB with human breast cell line and macrophage cell line and mouse monocytes
no band at 150 kDa, but tag shows at right MW
sees only bands at 90 kDa or lower
Ab: 1/500, 1/1000 o/n 4 degree and couple h at RT
block: milk or BSA
2nd: A...

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free of charge replacement with ab3425

Hello,

I am working with your RIP140 ChIP-grade antibody ab42126. In the specs, you say that it recognizes the C-terminal domain of the protein.

Do you have any more specific information? I would like to know if the antibody was raise...

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Thank you for contacting us. This antibody was generate from an 18 aa peptide from the C terminal.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

We have a question about one of your product, ab42126. We would like to know where this product is made, such as China, India or US. Would you please kindly provide us this information?

Thank you for contacting us.

This product's country of origin is the United States.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use o...

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