Recombinant fragment, corresponding to amino acids 431-541 of Human RIP2
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab155529 as a replacement.
Our Abpromise guarantee covers the use of ab57954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.|
|IHC-P||Use a concentration of 3 µg/ml.|
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded sections) human pancreas tissue labeling RIPK2 using ab57954 at 3 ug/ml.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RIP2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Ramos cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab57954 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab57954 was shown to recognize RIP2 when RIP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab57954 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.