• Product nameAnti-RIP2 antibody
    See all RIP2 primary antibodies
  • Description
    Mouse monoclonal to RIP2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 431-541 of Human RIP2



Our Abpromise guarantee covers the use of ab57954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 3 µg/ml.


  • FunctionActivates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
  • Tissue specificityDetected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 CARD domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD 3 antibody
    • CARD carrying kinase antibody
    • CARD containing ICE associated kinase antibody
    • CARD containing IL 1 beta ICE kinase antibody
    • CARD containing IL1 beta ICE kinase antibody
    • CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody
    • CARD containing interleukin 1 beta converting enzyme associated kinase antibody
    • CARD-containing IL-1 beta ICE-kinase antibody
    • CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody
    • CARD3 antibody
    • CARDIAK antibody
    • CCK antibody
    • CLARP kinase antibody
    • GIG 30 antibody
    • GIG30 antibody
    • Growth inhibiting gene 30 antibody
    • Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase antibody
    • Receptor interacting protein 2 antibody
    • Receptor interacting serine threonine kinase 2 antibody
    • Receptor interacting serine/threonine protein kinase 2 antibody
    • Receptor-interacting protein 2 antibody
    • Receptor-interacting serine/threonine-protein kinase 2 antibody
    • RICK antibody
    • RIP 2 antibody
    • RIP like interacting CLARP kinase antibody
    • RIP-2 antibody
    • RIP-like-interacting CLARP kinase antibody
    • RIPK 2 antibody
    • Ripk2 antibody
    • RIPK2_HUMAN antibody
    • TNFRSF antibody
    • Tyrosine-protein kinase RIPK2 antibody
    • UNQ277/PRO314/PRO34092 antibody
    see all

Anti-RIP2 antibody images

  • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded sections) human pancreas tissue labeling RIPK2 using ab57954 at 3 ug/ml.

  • Predicted band size : 61 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RIP2 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Ramos cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab57954 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab57954 was shown to recognize RIP2 when RIP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab57954 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Predicted band size : 61 kDa
    RIP2 antibody (ab57954) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-RIP2 antibody (ab57954)

This product has been referenced in:
  • Qian YJ  et al. Cigarette Smoke Modulates NOD1 Signal Pathway and Human ß Defensins Expression in Human Oral Mucosa. Cell Physiol Biochem 36:457-73 (2015). Human . Read more (PubMed: 25968832) »
  • Madrigal AG  et al. Pathogen-mediated proteolysis of the cell death regulator RIPK1 and the host defense modulator RIPK2 in human aortic endothelial cells. PLoS Pathog 8:e1002723 (2012). WB ; Human . Read more (PubMed: 22685397) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C

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Submitted Dec 11 2012