Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: RIP2 knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: Ramos cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab57954 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa. ab57954 was shown to recognize RIP2 when RIP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab57954 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - RIP2 antibody (ab57954)
Predicted band size : 61 kDa RIP2 antibody (ab57954) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-RIP2 antibody (ab57954)
This product has been referenced in:
Madrigal AG et al. Pathogen-mediated proteolysis of the cell death regulator RIPK1 and the host defense modulator RIPK2 in human aortic endothelial cells. PLoS Pathog8:e1002723 (2012).
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