Overview

  • Product name
  • Description
    Mouse monoclonal to RIP2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 431-541 of Human RIP2

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab155529 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab57954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 3 µg/ml.

Target

  • Function
    Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
  • Tissue specificity
    Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 CARD domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD 3 antibody
    • CARD carrying kinase antibody
    • CARD containing ICE associated kinase antibody
    • CARD containing IL 1 beta ICE kinase antibody
    • CARD containing IL1 beta ICE kinase antibody
    • CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody
    • CARD containing interleukin 1 beta converting enzyme associated kinase antibody
    • CARD-containing IL-1 beta ICE-kinase antibody
    • CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody
    • CARD3 antibody
    • CARDIAK antibody
    • CCK antibody
    • CLARP kinase antibody
    • GIG 30 antibody
    • GIG30 antibody
    • Growth inhibiting gene 30 antibody
    • Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase antibody
    • Receptor interacting protein 2 antibody
    • Receptor interacting serine threonine kinase 2 antibody
    • Receptor interacting serine/threonine protein kinase 2 antibody
    • Receptor-interacting protein 2 antibody
    • Receptor-interacting serine/threonine-protein kinase 2 antibody
    • RICK antibody
    • RIP 2 antibody
    • RIP like interacting CLARP kinase antibody
    • RIP-2 antibody
    • RIP-like-interacting CLARP kinase antibody
    • RIPK 2 antibody
    • Ripk2 antibody
    • RIPK2_HUMAN antibody
    • TNFRSF antibody
    • Tyrosine-protein kinase RIPK2 antibody
    • UNQ277/PRO314/PRO34092 antibody
    see all

Images

  • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded sections) human pancreas tissue labeling RIPK2 using ab57954 at 3 ug/ml.



  • Predicted band size : 61 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RIP2 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Ramos cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab57954 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab57954 was shown to recognize RIP2 when RIP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab57954 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 61 kDa
    RIP2 antibody (ab57954) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References

This product has been referenced in:
  • Qian YJ  et al. Cigarette Smoke Modulates NOD1 Signal Pathway and Human ß Defensins Expression in Human Oral Mucosa. Cell Physiol Biochem 36:457-73 (2015). Human . Read more (PubMed: 25968832) »
  • Madrigal AG  et al. Pathogen-mediated proteolysis of the cell death regulator RIPK1 and the host defense modulator RIPK2 in human aortic endothelial cells. PLoS Pathog 8:e1002723 (2012). WB ; Human . Read more (PubMed: 22685397) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Dec 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up