Our Abpromise guarantee covers the use of ab56164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human RIP3 peptide (ab178834). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 3 - 12 µg/ml.|
This image is courtesy of an anonymous Abreview
Blocked with 5% milk for 1 hour at 25C
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human pancreas sections labelling RIP3 with ab56164 at 3 - 12 µg/mL. The secondary antibody used was a donkey anti-rabbit antibody at 1/200. The exposure time was 0.2 seconds at magnification of 20X.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"