Overview

  • Product nameAnti-RIP3 antibody
    See all RIP3 primary antibodies
  • Description
    Rabbit polyclonal to RIP3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee
  • Immunogen

    Synthetic peptide within Human RIP3 aa 480-510. The exact sequence is proprietary.
    Database link: Q9Y578
    (Peptide available as ab204450)

  • Positive control
    • NIH3T3 cell lysate; Paraffin embedded human pancreatic tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab72106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionPromotes apoptosis.
  • Tissue specificityDetected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Anti-RIP3 antibody images

  • Immunohistochemical analysis of RIP3 expression in paraffin embedded human pancreatic tissue using ab72106 at 10ug/ml.

References for Anti-RIP3 antibody (ab72106)

This product has been referenced in:
  • Omoto S  et al. Suppression of RIP3-dependent necroptosis by human cytomegalovirus. J Biol Chem 290:11635-48 (2015). Read more (PubMed: 25778401) »
  • Zhao J  et al. Mixed lineage kinase domain-like is a key receptor interacting protein 3 downstream component of TNF-induced necrosis. Proc Natl Acad Sci U S A 109:5322-7 (2012). WB ; Human . Read more (PubMed: 22421439) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (HT-29 Human adenocarcinoma)
Specification HT-29 Human adenocarcinoma
Treatment 48 hrs, DMEM no serum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 22°C
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Submitted Feb 05 2014

Merci pour votre patience.

Notre recherche interne donnais comme seul candidat l'anticorpsab72106 qui pourrait avoir été utilisé dans la publication que vous nous avez cité. Malheureusement est il très peu p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"