Overview

  • Product name
  • Description
    Rabbit polyclonal to RIP3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee
  • Immunogen

    Synthetic peptide within Human RIP3 aa 480-510. The exact sequence is proprietary.
    Database link: Q9Y578
    (Peptide available as ab204450)

  • Positive control
    • NIH3T3 cell lysate; Paraffin embedded human pancreatic tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab72106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Promotes apoptosis.
  • Tissue specificity
    Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of RIP3 expression in paraffin embedded human pancreatic tissue using ab72106 at 10ug/ml.

References

This product has been referenced in:
  • Zhou X  et al. The bile acid receptor FXR attenuates acinar cell autophagy in chronic pancreatitis. Cell Death Discov 3:17027 (2017). WB ; Human . Read more (PubMed: 28660075) »
  • Zhang Y  et al. RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome. Nat Commun 8:14329 (2017). Read more (PubMed: 28176780) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (HT-29 Human adenocarcinoma)
Specification
HT-29 Human adenocarcinoma
Treatment
48 hrs, DMEM no serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2014

Merci pour votre patience.

Notre recherche interne donnais comme seul candidat l'anticorpsab72106 qui pourrait avoir été utilisé dans la publication que vous nous avez cité. Malheureusement est il très peu p...

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Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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