Overview

  • Product name
  • Description
    Rabbit polyclonal to RIPX
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide derived from a region between amino acids 414-463 of human RIPX. (Note: exact amino acid sequence is proprietary).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab30986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes.
  • Sequence similarities
    Contains 1 RUN domain.
  • Post-translational
    modifications
    Isoform 1 is partially phosphorylated.
  • Cellular localization
    Cell projection. Enriched in F-actin enriched filopodia and lamellipodia of growth cones of axons and minor processes of hippocampal neurons in culture.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein RUFY3 antibody
    • rap2 interacting protein x antibody
    • Rap2 interacting protein x isoform 2 antibody
    • Rap2-interacting protein x antibody
    • RIPx antibody
    • Rpipx antibody
    • Rufy3 antibody
    • RUFY3_HUMAN antibody
    • RUN and FYVE domain containing 3 antibody
    • Singar antibody
    • Singar1 antibody
    • Single axon regulated protein antibody
    • single axon related 1 antibody
    • Single axon-regulated protein antibody
    see all

Images

  • Anti-RIPX antibody (ab30986) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkay cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

References

ab30986 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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