Overview

  • Product nameAnti-RIT1 antibody
    See all RIT1 primary antibodies
  • Description
    Rabbit polyclonal to RIT1
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab53720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.

Target

  • FunctionPlays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.
  • Tissue specificityExpressed in many tissues.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTP binding protein Roc1 antibody
    • GTP-binding protein Rit1 antibody
    • NS8 antibody
    • Ras like protein expressed in many tissues antibody
    • Ras like without CAAX 1 antibody
    • Ras-like protein expressed in many tissues antibody
    • Ras-like without CAAX protein 1 antibody
    • RIBB antibody
    • Ric like expressed in many tissues antibody
    • Ric-like protein without CAAX motif 1 antibody
    • RIT antibody
    • RIT1 antibody
    • RIT1_HUMAN antibody
    • ROC1 antibody
    see all

Anti-RIT1 antibody images

  • ICC/IF image of ab53720 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RIT1 antibody (ab53720)

ab53720 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The immunogen is from the C terminus. Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"