Overview

  • Product nameAnti-RIT2 antibody [1G2]
    See all RIT2 primary antibodies
  • Description
    Mouse monoclonal [1G2] to RIT2
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of Human RIT2 produced in HEK293T cells (NP_002921).

  • Positive control
    • HEK293T cells were transfected with RIT2, COS7 cells transiently transfected with RIT2
  • General notesConcentration - 0.5~1.0 mg/ml (Lot Dependent).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number1G2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionBinds and exchanges GTP and GDP.
  • Tissue specificityNeuron-specific.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTP binding protein Rit2 antibody
    • GTP binding protein Roc2 antibody
    • GTP-binding protein Rit2 antibody
    • Ras like protein expressed in neurons antibody
    • Ras like without CAAX 2 antibody
    • Ras like without CAAX protein 2 antibody
    • Ras-like protein expressed in neurons antibody
    • Ras-like without CAAX protein 2 antibody
    • RIBA antibody
    • Ric (Drosophila) like antibody
    • Ric like antibody
    • Ric like, expressed in neurons antibody
    • RIN antibody
    • RIT2 antibody
    • RIT2_HUMAN antibody
    • ROC2 antibody
    see all

Anti-RIT2 antibody [1G2] images

  • All lanes : Anti-RIT2 antibody [1G2] (ab119082) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY RIT2

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 25 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205367)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIT2 using ab119082 at a dilution of 1/100.
  • HEK293T cells transfected with either RC205367 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119082 at a dilution of 1/100, and then analyzed by flow cytometry.

References for Anti-RIT2 antibody [1G2] (ab119082)

ab119082 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119082.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"