The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Tubulin-binding protein that acts as a negative regulator of Notch signaling pathway. Shuttles between the cytoplasm and the nucleus and mediates the nuclear export of RBPJ/RBPSUH, thereby preventing the interaction between RBPJ/RBPSUH and NICD product of Notch proteins (Notch intracellular domain), leading to down-regulate Notch-mediated transcription. May play a role in neurogenesis.
Belongs to the RITA family.
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. Shuttles rapidly between the cytoplasm and the nucleus. The function of centrosome localization is still unclear.
Immunohistochemical staining of RITA in paraffin embedded Human rectum tissue by Immunohistochemistry, using ab122531 at a 1/75 dilution. The image shows cytoplasmic and membranous positivity in glandular cells.