Overview

  • Product nameAnti-RIZ1 antibody
    See all RIZ1 primary antibodies
  • Description
    Rabbit polyclonal to RIZ1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ChIP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human PRDM2/Riz1.

    (Peptide available as ab23566.)

  • Positive control
    • This antibody gave a positive signal in MCF7 whole cell lysate + Gal4 C-terminal tagged PRDM2/Riz1.
  • General notes


    The retinoblastoma protein is a target of viral oncoproteins. To explore the hypothesis that viral proteins may be structural mimics of cellular RB-binding proteins that normally mediate RB function, Buyse et al. (1995) searched cDNA libraries for RB-binding proteins. They reported the cloning of a cDNA for the zinc finger protein they called RIZ from rat and human cells. It is a 250-kD nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. While the function of RIZ was not clear, its structure and pattern of expression suggested to Buyse et al. (1995) a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab9710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa.
  • Application notesIs unsuitable for ChIP.
  • Target

    • FunctionS-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
    • Tissue specificityHighly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
    • Sequence similaritiesContains 8 C2H2-type zinc fingers.
      Contains 1 SET domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • GATA-3-binding protein G3B antibody
      • HUMHOXY1 antibody
      • KMT8 antibody
      • Lysine N-methyltransferase 8 antibody
      • MTB-ZF antibody
      • MTE-binding protein antibody
      • PR domain containing 2, with ZNF domain antibody
      • PR domain zinc finger protein 2 antibody
      • PR domain-containing protein 2 antibody
      • PRDM2 antibody
      • PRDM2_HUMAN antibody
      • Retinoblastoma protein binding zinc finger protein antibody
      • Retinoblastoma protein-interacting zinc finger protein antibody
      • Retinoblastoma protein-interacting zinc-finger protein antibody
      • RIZ antibody
      • RIZ1 antibody
      • RIZ2 antibody
      • Zinc finger DNA binding protein antibody
      • Zinc finger protein RIZ antibody
      see all

    Anti-RIZ1 antibody images

    • Anti-RIZ1 antibody (ab9710) at 1/1000 dilution

      Secondary
      Goat anti-Rabbit at 1/25000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

      This image is courtesy of Karen Halls, University of Cambridge

      MCF7 cells were transfected with 2µg Gal4 C-terminal tagged RIZ1 (aa 2-222 from Rat)

    References for Anti-RIZ1 antibody (ab9710)

    This product has been referenced in:
    • Congdon LM  et al. The PR-Set7 binding domain of Riz1 is required for the H4K20me1-H3K9me1 trans-tail 'histone code' and Riz1 tumor suppressor function. Nucleic Acids Res 42:3580-9 (2014). WB ; Human . Read more (PubMed: 24423864) »
    • Kera Y  et al. Methionine adenosyltransferase II-dependent histone H3K9 methylation at the COX-2 gene locus. J Biol Chem 288:13592-601 (2013). Mouse . Read more (PubMed: 23539621) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Spinal Cord)
    Specification Spinal Cord
    Fixative Paraformaldehyde
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Sep 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"