Overview

  • Product nameAnti-RLA DQ antibody [2C4] (FITC)
    See all RLA DQ primary antibodies
  • Description
    Mouse monoclonal [2C4] to RLA DQ (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab25685 reacts with RLA-DQ-transfected cells, but not with RLA-DR-transfected cells.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Rabbit
  • Immunogen

    Tissue/ cell preparation (Rabbit) (spleen cells)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number2C4
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • RelevanceIn normal adult rabbits, this antibody reacts with 30-60% of peripheral blood leukocytes. It has also been reported that the antibody stains: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells in inflamed synovium. DQ? transcripts have been detected in bone marrow and spleen and, to a lesser extent, in appendix, lymph nodes, and other organs.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • MHC class II antigen [Fragment] antibody
    • MHC class II antigen antibody
    • RLA DQA antibody

References for Anti-RLA DQ antibody [2C4] (FITC) (ab25685)

This product has been referenced in:
  • Wilkinson JM  et al. Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit. J Pathol 170:315-20 (1993). Read more (PubMed: 8133406) »
  • Spieker-Polet H  et al. Rabbit major histocompatibility complex. IV. Expression of major histocompatibility complex class II genes. J Immunogenet 17:123-32 (1990). Read more (PubMed: 2120349) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"