The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells.
Use at an assay dependent dilution. Fix with acetone.
Use at an assay dependent dilution.
RelevanceIn normal adult rabbits, this antibody reacts with 30-60% of peripheral blood leukocytes. It has also been reported that the antibody stains: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells in inflamed synovium. DQ? transcripts have been detected in bone marrow and spleen and, to a lesser extent, in appendix, lymph nodes, and other organs.