Overview

  • Product name
  • Description
    Rabbit polyclonal to RLF
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1850 to the C-terminus of Human RLF.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysate, as well as wild-type Mouse embryo head tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa).

Target

Images

  • Anti-RLF antibody (ab115011) at 1/500 dilution + Wild-type Mouse Embryo Head tissue at 20 µg

    Secondary
    Anti-Rabbit HRP Conjugated
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 218 kDa
    Observed band size : 280 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa (possible cross reactivity).
  • All lanes : Anti-RLF antibody (ab115011) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit HRP Conjugated

    Predicted band size : 218 kDa
    Observed band size : 265 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 135 kDa (possible cross reactivity),150 kDa (possible cross reactivity).

References

This product has been referenced in:
  • Harten SK  et al. The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers. BMC Biol 13:21 (2015). WB . Read more (PubMed: 25857663) »
  • Daxinger L  et al. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse. Genome Biol 14:R96 (2013). Read more (PubMed: 24025402) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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