Anti-RLF antibody (ab115154)
Key features and details
- Rabbit polyclonal to RLF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RLF antibody -
Description
Rabbit polyclonal to RLF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 500-550 of Human RLF (Q13129).
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Positive control
- HeLa and 293T cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 218 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 218 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May be involved in transcriptional regulation. -
Tissue specificity
Widely expressed in fetal and adult tissues. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 14 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6018 Human
- Entrez Gene: 109263 Mouse
- Entrez Gene: 313566 Rat
- Omim: 180610 Human
- SwissProt: Q13129 Human
- Unigene: 205627 Human
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Alternative names
- Rearranged L-myc fusion gene protein antibody
- RLF antibody
- RLF_HUMAN antibody
see all
Images
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All lanes : Anti-RLF antibody (ab115154) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Predicted band size: 218 kDa
Exposure time: 3 minutes -
Immunoprecipitation of RLF (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-RLF antibody (ab115154); Lane 2- Immunoprecipitation with IgG control. Anti-RLF antibody (ab115154) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab115154 has not yet been referenced specifically in any publications.