Overview

  • Product name
  • Description
    Rabbit polyclonal to RLF
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 500-550 of Human RLF (Q13129).

  • Positive control
    • HeLa and 293T cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 218 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-RLF antibody (ab115154) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg


    Predicted band size : 218 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of RLF (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-RLF antibody (ab115154); Lane 2- Immunoprecipitation with IgG control. Anti-RLF antibody (ab115154) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.

References

ab115154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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