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Rabbit polyclonal to RLF
Predicted to work with:
Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 500-550 of Human RLF (Q13129).
HeLa and 293T cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 218 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in transcriptional regulation.
Widely expressed in fetal and adult tissues.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 14 C2H2-type zinc fingers.
Information by UniProt
Rearranged L-myc fusion gene protein antibody
Western blot - RLF antibody (ab115154)
All lanes : Anti-RLF antibody (ab115154) at 0.1 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : 293T cell lysate at 50 µg Predicted band size: 218 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-RLF antibody (ab115154)
Immunoprecipitation of RLF (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-RLF antibody (ab115154); Lane 2- Immunoprecipitation with IgG control.
Anti-RLF antibody (ab115154) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"