Overview

  • Product name
  • Description
    Rabbit polyclonal to RMI2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RMI2.

  • Positive control
    • This antibody gave a positive signal in both HEK293 and MCF7 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab108491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

Target

  • Function
    Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
  • Sequence similarities
    Belongs to the RMI2 family.
    Contains 1 OB DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated during mitosis.
  • Cellular localization
    Nucleus. Colocalizes with BLM at nuclear DNA repair foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLAP18 antibody
    • BLM-associated protein of 18 kDa antibody
    • C16orf75 antibody
    • hRMI2 antibody
    • MGC24665 antibody
    • RecQ-mediated genome instability protein 2 antibody
    • RMI2 antibody
    • RMI2_HUMAN antibody
    see all

Images

  • All lanes : Anti-RMI2 antibody (ab108491) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab108491 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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