Overview

  • Product nameAnti-RMI2 antibody
    See all RMI2 primary antibodies
  • Description
    Rabbit polyclonal to RMI2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GKYVMVMGVV QACSPEPCLQ AVKMTDLSDN PIHESMWELE VEDLHRNI, corresponding to amino acids 99-146 of Human RMI2.

  • Positive control
    • Human stomach tissue; RT 4, U 251 MG and Human liver lysates; U 251 MG cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionEssential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
  • Sequence similaritiesBelongs to the RMI2 family.
    Contains 1 OB DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated during mitosis.
  • Cellular localizationNucleus. Colocalizes with BLM at nuclear DNA repair foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLAP18 antibody
    • BLM-associated protein of 18 kDa antibody
    • C16orf75 antibody
    • hRMI2 antibody
    • MGC24665 antibody
    • RecQ-mediated genome instability protein 2 antibody
    • RMI2 antibody
    • RMI2_HUMAN antibody
    see all

Anti-RMI2 antibody images

  • ab122685, at 1/200 dilution, staining RMI2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • ab122685, at 4 µg/ml staining RIM2 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
  • All lanes : Anti-RMI2 antibody (ab122685) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References for Anti-RMI2 antibody (ab122685)

ab122685 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"