Anti-RMND1 antibody (ab223119)
Key features and details
- Rabbit polyclonal to RMND1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RMND1 antibody -
Description
Rabbit polyclonal to RMND1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human RMND1 aa 35-160.
Database link: Q9NWS8 -
Positive control
- WB: MCF7 and HEK-293 cell lysates; Mouse skeletal muscle lysate. IHC-P: Human adrenal gland tissue and ovarian cancer tissues. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Function
Required for mitochondrial translation, possibly by coordinating the assembly or maintenance of the mitochondrial ribosome (PubMed:23022098, PubMed:25604853). -
Involvement in disease
Combined oxidative phosphorylation deficiency 11 -
Sequence similarities
Belongs to the RMD1/sif2 family. -
Cellular localization
Mitochondrion. May be localized in mitochondrial RNA granules (PubMed:25604853). - Information by UniProt
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Database links
- Entrez Gene: 55005 Human
- Entrez Gene: 66084 Mouse
- SwissProt: Q9NWS8 Human
- SwissProt: Q8CI78 Mouse
- Unigene: 486835 Human
- Unigene: 744622 Human
- Unigene: 153111 Mouse
- Unigene: 313137 Mouse
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Alternative names
- bA351K16 antibody
- bA351K16.3 antibody
- C6orf96 antibody
see all
Images
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MCF7 (human breast adenocarcinoma cell line) cells stained for RMND1 (green) using ab223119 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human ovarian cancer tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human adrenal gland tissue stained for RMND1 using ab223119 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-RMND1 antibody (ab223119) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : Mouse skeletal muscle lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab223119 has been referenced in 1 publication.
- Key J et al. CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2. Cells 12:N/A (2022). PubMed: 36611846