The RNA Bisulfite Conversion Kit (ab185911) is a complete set of optimized reagents required for fast bisulfite conversion on a RNA sample. The converted RNA obtained with the RNA Bisulfite Conversion Kit is suitable for various downstream RNA methylation analysis including methylation specific RT-PCR, MS-HRM, and bisulfite RNA-sequencing (e.g., pyrosequencing and deep-sequencing).
DNA cytosine methylation occurs by the covalent addition of a methyl group at the 5-carbon of the cytosine ring by DNA methyltransferases, resulting in 5-methylcytosine (5-mC). This process has been well studied and is generally associated with repression of gene expression. It was also observed that in humans, 5-mC occurs in various RNA molecules including tRNAs, rRNAs, mRNAs and non-coding RNAs (ncRNAs). At least 10,275 5-mC candidate sites were discovered in mRNAs and ncRNAs, which covered 10.6% of the total cytosine residues in the transcriptome. 5-mC seems to be enriched in some classes of ncRNA, but relatively depleted in mRNAs. However, the majority (83%) of their candidate sites were found in mRNAs. Within these transcripts 5-mC appears to be depleted within protein coding sequences but enriched in 5’ and 3’ UTRs. Two different methyltransferases, NSUN2 and Dnmt2 are known to catalyze the 5-mC modification in eukaryotic RNAs. Recent data strongly suggest that RNA cytosine methylation affects the regulation of various biological processes such as RNA stability and mRNA translation. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Thus, impaired processing of vault ncRNA may contribute to the etiology of human disorders related to NSUN2-deficiency.
|Control Primer F||1 x 10µl|
|Control Primer R||1 x 10µl|
|Conversion Buffer||1 x 8ml|
|Conversion Powder||1 x 5 vials|
|Desulphonation Solution||1 x 300µl|
|Elution Buffer||1 x 1.2ml|
|F-Collection Tube||1 x 50 units|
|F-Spin Column||1 x 50 units|
|RNA Binding Solution||1 x 13ml|
ab185911 has not yet been referenced specifically in any publications.