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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-RNA Helicase A antibody
See all RNA Helicase A products (8) ...
Description
Rabbit polyclonal to RNA Helicase A
Tested applications
IP, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human, African Green Monkey
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human RNA Helicase A.
(Peptide available as ab27786.)
Positive control
HeLa whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
ICC/IF: 1/1000.
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at 2 µg/mg of lysate (see Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Domain
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
Post-translational
modifications
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
Target information above from: UniProt accessionQ08211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
Database links
- Entrez Gene: 281115 Cow
- Entrez Gene: 1660 Human
- Entrez Gene: 13211 Mouse
- Omim: 603115 Human
- SwissProt: Q28141 Cow
- SwissProt: Q08211 Human
- SwissProt: O70133 Mouse
- Unigene: 191518 Human
see all
Anti-RNA Helicase A antibody images:
Western blot - RNA Helicase A antibody (ab26271)
Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 141 kDa
Observed band size : 141 kDa
Immunocytochemistry/ Immunofluorescence - RNA Helicase A antibody (ab26271)
ab26271, at 1/1000 dilution, staining Human RNA Helicase A in HeLa cells by immunofluorescence. Secondary antibody: Goat anti-Rabbit conjugated to Cy3; Counter-Stain: DAPI;
This image is courtesy of an Abreview submitted by Dr Kirk McManus
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RNA Helicase A antibody (ab26271)
Image courtesy of Human Protein Atlas . ab26271 staining RNA helicase A in human stomach, showing a predominantly nuclear staining pattern in glandular cells. Paraffin embedded human stomach tissue was incubated with ab26271 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab26271 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
References for Anti-RNA Helicase A antibody (ab26271)
This product has been referenced in:
- Georges Aet al. SUMOylation of the Forkhead Transcription Factor FOXL2 Promotes Its Stabilization/Activation through Transient Recruitment to PML Bodies. PLoS One 6:e25463 (2011). ICC/IF.Read more (PubMed: 22022399) »
- Sadler AJet al. An antiviral response directed by PKR phosphorylation of the RNA helicase A. PLoS Pathog 5:e1000311 (2009). WB; Human.Read more (PubMed: 19229320) »
See all 3 publications for this product
Publishing research using ab26271? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"