The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionUnwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. Contains 2 DRBM (double-stranded RNA-binding) domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
DomainThe MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
Post-translational modificationsMethylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization. May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
DEAD/H box 9 antibody
DEAD/H box polypeptide 9 antibody
DEAH (Asp Glu Ala His) box polypeptide 9 antibody
DEAH box polypeptide 9 antibody
DEAH box protein 9 antibody
DHX 9 antibody
NDH 2 antibody
NDH II antibody
Nuclear DNA helicase II antibody
Anti-RNA Helicase A antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody (ab70777)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RNA Helicase A with ab70777 at 1/200 (1µg/ml). Detection: DAB.
Western blot - RNA Helicase A antibody (ab70777)
All lanes : Anti-RNA Helicase A antibody (ab70777) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 141 kDa Observed band size : 141 kDa
Exposure time : 10 seconds
Immunoprecipitation - RNA Helicase A antibody (ab70777)
Detection of RNA Helicase A by Western Blot of Immunprecipitate.
ab70777 at 1µg/ml staining RNA Helicase A in HeLa whole cell lysate immunoprecipitated using ab70777 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-RNA Helicase A antibody (ab70777)