Overview

  • Product name
    Anti-RNA Helicase A antibody
    See all RNA Helicase A primary antibodies
  • Description
    Goat polyclonal to RNA Helicase A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Pufferfish, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide:

    TEGRNALIHKSSVNC

    , corresponding to internal sequence amino acids 1015-1029 of human RNA Helicase A (NP_001348.2).

  • Positive control
    • HeLa nuclear lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 141 kDa).

Target

  • Function
    Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
  • Post-translational
    modifications
    Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase A antibody
    • ATP-dependent RNA helicase A antibody
    • DDX 9 antibody
    • DDX9 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
    • DEAD/H box 9 antibody
    • DEAD/H box polypeptide 9 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
    • DEAH box polypeptide 9 antibody
    • DEAH box protein 9 antibody
    • DHX 9 antibody
    • dhx9 antibody
    • DHX9_HUMAN antibody
    • Leukophysin antibody
    • LKP antibody
    • NDH 2 antibody
    • NDH II antibody
    • NDH2 antibody
    • NDHII antibody
    • Nuclear DNA helicase II antibody
    • RHA antibody
    see all

Images

  • Anti-RNA Helicase A antibody (ab87608) at 1 µg/ml + HeLa nuclear lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 141 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

References

ab87608 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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