Recombinant
RabMAb

Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189)

Overview

  • Product name
    Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal
    See all RNA Helicase A primary antibodies
  • Description
    Rabbit monoclonal [EPR13520(B)] to RNA Helicase A - N-terminal
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RNA Helicase A aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q08211

  • Positive control
    • BxPC-3, Jurkat, HeLa and 293T cell lysates; 293T cells; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab180189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 141 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DEAH subfamily.
      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Domain
      The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
    • Post-translational
      modifications
      Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
      May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase A antibody
      • ATP-dependent RNA helicase A antibody
      • DDX 9 antibody
      • DDX9 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
      • DEAD/H box 9 antibody
      • DEAD/H box polypeptide 9 antibody
      • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
      • DEAH box polypeptide 9 antibody
      • DEAH box protein 9 antibody
      • DHX 9 antibody
      • dhx9 antibody
      • DHX9_HUMAN antibody
      • Leukophysin antibody
      • LKP antibody
      • NDH 2 antibody
      • NDH II antibody
      • NDH2 antibody
      • NDHII antibody
      • Nuclear DNA helicase II antibody
      • RHA antibody
      see all

    Images

    • All lanes : Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189) at 1/1000 dilution

      Lane 1 : BxPC-3 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 141 kDa
    • Immunofluorescent analysis of 293T cells labeling RNA Helicase A with ab180189 at 1/100.

    • Flow Cytometrical analysis of permeabilized Jurkat cells labeling RNA Helicase A with ab180189 at 1/10 (red) or a rabbit IgG negative (green).

    References

    ab180189 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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