Recombinant
RabMAb

Anti-RNA Helicase A antibody [EPR13521] (ab183731)

Overview

  • Product name
    Anti-RNA Helicase A antibody [EPR13521]
    See all RNA Helicase A primary antibodies
  • Description
    Rabbit monoclonal [EPR13521] to RNA Helicase A
  • Tested applications
    Suitable for: IHC-P, WB, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RNA Helicase A aa 1150-1250. The exact sequence is proprietary.
    Database link: Q08211

  • Positive control
    • HeLa, Jurkat, 293 or BxPC-3 lysate, Human lung adenocarcinoma and mouse colon tissue, HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/10000 - 1/50000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
ICC 1/250.
Flow Cyt 1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
  • Post-translational
    modifications
    Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase A antibody
    • ATP-dependent RNA helicase A antibody
    • DDX 9 antibody
    • DDX9 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
    • DEAD/H box 9 antibody
    • DEAD/H box polypeptide 9 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
    • DEAH box polypeptide 9 antibody
    • DEAH box protein 9 antibody
    • DHX 9 antibody
    • dhx9 antibody
    • DHX9_HUMAN antibody
    • Leukophysin antibody
    • LKP antibody
    • NDH 2 antibody
    • NDH II antibody
    • NDH2 antibody
    • NDHII antibody
    • Nuclear DNA helicase II antibody
    • RHA antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Mouse colon tissue labeling RNA Helicase A with ab183731 at a 1/100 dilution. Prediluted HRP conjugated Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • Flow cytometry analysis of HeLa cells using ab183731 at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Immunocytochemistry analysis of HeLa cells (fixative -20℃ Acetone) labeling RNA Helicase A with ab183731 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.

  • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labeling RNA Helicase A with ab183731 at a 1/100 dilution. Prediluted HRP conjugated Rabbit IgG secondary used. Counterstained with Hematoxylin.

  • All lanes : Anti-RNA Helicase A antibody [EPR13521] (ab183731) at 1/50000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Jurkat lysate
    Lane 3 : 293 lysate
    Lane 4 : BxPC-3 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 141 kDa

References

ab183731 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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