Anti-RNA polymerase beta antibody [EPR18704] (ab191598)
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- Specific References
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Overview
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Product nameAnti-RNA polymerase beta antibody [EPR18704]
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DescriptionRabbit monoclonal [EPR18704] to RNA polymerase beta
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WBmore details
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Species reactivityReacts with: Escherichia coli
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Escherichia coli RNA polymerase beta aa 1300 to the C-terminus. The exact sequence is proprietary.
Database link: P0A8V2 -
Positive control
- WB & IP: E.coli whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR18704
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IsotypeIgG
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Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab191598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/30. | |
| WB | 1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
Target
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RelevanceRNA polymerase beta (rpoB) is the ß subunit of the bacterial RNA polymerase and is the subunit which possesses the polymerase activity. This protein is a highly conserved enzyme contained by many bacteria and has been reported as a useful marker for studies in bacetrial identification and ecology.
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Cellular localizationCell Membrane and Cytoplasmic
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Database links
- Entrez Gene: 948488 Escherichia coli
- SwissProt: P0A8V2 Escherichia coli
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Alternative names
- groN antibody
- nitB antibody
- rif antibody
see all
Images
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![Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](http://a.abcam.com/ps/products/191/ab191598/Images/ab191598-246642-wb.jpg)
Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)Anti-RNA polymerase beta antibody [EPR18704] (ab191598) at 1/2000 dilution + E.coli whole cell lysates at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](http://a.abcam.com/ps/products/191/ab191598/Images/ab191598-246641-ip.jpg)
Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: E.coli whole cell lysates 10ug (Input). Lane 2: ab191598 IP in E.coli whole cell lysates. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
References
ab191598 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"