Recombinant
RabMAb

Anti-RNA polymerase beta antibody [EPR18704] (ab191598)

Overview

  • Product name
    Anti-RNA polymerase beta antibody [EPR18704]
  • Description
    Rabbit monoclonal [EPR18704] to RNA polymerase beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Escherichia coli RNA polymerase beta aa 1300 to the C-terminus. The exact sequence is proprietary.
    Database link: P0A8V2

  • Positive control
    • WB & IP: E.coli whole cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab191598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).

Target

  • Relevance
    RNA polymerase beta (rpoB) is the ß subunit of the bacterial RNA polymerase and is the subunit which possesses the polymerase activity. This protein is a highly conserved enzyme contained by many bacteria and has been reported as a useful marker for studies in bacetrial identification and ecology.
  • Cellular localization
    Cell Membrane and Cytoplasmic
  • Database links
    • Alternative names
      • groN antibody
      • nitB antibody
      • rif antibody
      • RNA pol beta antibody
      • RNA polymerase subunit beta antibody
      • RNA polymerase, beta subunit antibody
      • RNAP subunit beta antibody
      • ron antibody
      • rpoB antibody
      • stl antibody
      • stv antibody
      • tabD antibody
      • Transcriptase subunit beta antibody
      see all

    Images

    • Anti-RNA polymerase beta antibody [EPR18704] (ab191598) at 1/2000 dilution + E.coli whole cell lysates at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 150 kDa
      Observed band size: 150 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

      Lane 1: E.coli whole cell lysates 10ug (Input). Lane 2: ab191598 IP in E.coli whole cell lysates. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

    References

    ab191598 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (Shigella flexneri 5a)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    8e+007 cells
    Specification
    Shigella flexneri 5a
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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