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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Escherichia coli RNA polymerase beta aa 1300 to the C-terminus. The exact sequence is proprietary.
Database link: P0A8V2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab191598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).|
Blocking/Dilution buffer: 5% NFDM/TBST.
RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: E.coli whole cell lysates 10ug (Input). Lane 2: ab191598 IP in E.coli whole cell lysates. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
ab191598 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"