RNA polymerase II CTD Panel (RNA pol II CTD, phospho S2, phospho S5) (ab103968)

Overview

  • Product nameRNA polymerase II CTD Panel (RNA pol II CTD, phospho S2, phospho S5)
  • Product overview

    ab103968 is a RNA polymerase II CTD Panel designed for the validation and characterization of the phosphorylation state of the carboxy-terminal domain (CTD) repeat YSPTSPS of RNA polymerase II. Numerous enzymes, including cell cycle-dependent kinases and TFIIF-dependent phosphatases target the CTD of RNA polymerase II, and the phosphorylation pattern changes during the transcription cycle. S5 phosphorylation occurs in promoter-proximal regions, while S2 phosphorylation predominates in regions that are more distal from the promoter during transcription elongation.

Properties

  • Alternative names
    • DNA directed RNA polymerase II A
    • DNA-directed RNA polymerase II subunit A
    • DNA-directed RNA polymerase II subunit RPB1
    • DNA-directed RNA polymerase III largest subunit
    • Polr2a
    • RNA polymerase II CTD repeat YSPTSPS
    • RNA polymerase II subunit B1
    • RNA-directed RNA polymerase II subunit RPB1
    • RPB1
    • RPB1_HUMAN
    see all

RNA polymerase II CTD Panel (RNA pol II CTD, phospho S2, phospho S5) images

  • Lanes 1, 3 & 4 : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131) at 1/500 dilution
    Lane 2 : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade (ab5131) at 1/2000 dilution

    Lane 1 : HeLa nuclear extract
    Lane 2 : HeLa nuclear extract
    Lane 3 : HeLa nuclear extract with Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488) at 1 µg/ml
    Lane 4 : HeLa nuclear extract with S. cerevisiae RNA polymerase II CTD repeat YSPTSPS peptide (ab12795) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
  • Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab5408 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 2µg of ab5095 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the inactive AFM and F8 promoters, a region in the GAPDH gene (active) and over the g-Actin gene (active). Schematic diagram of the g-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.
  • Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 mins. The ChIP was performed with 25 µg of chromatin, 2 µg of ab5131 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the inactive AFM and F8 promoters, the GAPDH promoter (active) and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

References for RNA polymerase II CTD Panel (RNA pol II CTD, phospho S2, phospho S5) (ab103968)

This product has been referenced in:
  • Patel M  et al. Tumor-specific retargeting of an oncogenic transcription factor chimera results in dysregulation of chromatin and transcription. Genome Res 22:259-70 (2012). ChIP ; Human . Read more (PubMed: 22086061) »
  • Ross-Innes CS  et al. Cooperative interaction between retinoic acid receptor-alpha and estrogen receptor in breast cancer. Genes Dev 24:171-82 (2010). Read more (PubMed: 20080953) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"